FLUORESCENCE AND BIOSYSTEMS

For more details about this research contact Prof. Manuel Prieto (manuel.prieto[AT]ist.utl.pt)

Luminescence of carbon-based nano-objects

  • Multifunctional luminescent platforms
  • TADF of fullerenes, PAH and related graphene-like species


Representative Publications:
  • Sensing and imaging of oxygen with ppb limits of detection and based on the quenching of the delayed fluorescence of 13C70 fullerene in polymer hosts, Analytical Chemistry 2013, 85, 1300. |www|
  • Controlled release of singlet oxygen using diphenylanthracene functionalized polymer nanoparticles, Chemical Communications 2014, 50, 3317. |www|
  • Temperature dependence of the phosphorescence and of the thermally activated delayed fluorescence of 12C70 and 13C70 in amorphous polymer matrices. Is a second triplet involved?, Methods and Applications in Fluorescence 2014, 2, 035002. |www|
  • Molecular Fluorescence: Principles and Applications, 2nd ed., 590 pp., Wiley-VCH, Weinheim, 2012 (softcover ed. 2013). |www|
  • Distinctive characteristics of the decay function for phosphorescence in the presence of radiative transfer, Journal of Mathematical Chemistry 2014, 52, 2271. |www|


Membrane Biophysics

  • Lipid phase diagrams and membrane rafts
  • Bioactive Lipids, Ceramides, Phosphatidylinositols
  • Bile acids


Representative Publications:
  • Changes in membrane biophysical properties induced by sphingomyelinase depend on the sphingolipid N-acyl chain. Journal of Lipid Research, 2014, 55(1), 53-61. |www|
  • Ca(2+) induces PI(4,5)P2 clusters on lipid bilayers at physiological PI(4,5)P2 and Ca(2+). Biochim Biophys Acta, 2014, 1838(3), 822-30. |www|
  • Influence of intracellular membrane pH on sphingolipid organization and membrane biophysical properties. Langmuir, 2014, 30(14):4094-104. |www|
  • Ceramide: a simple sphingolipid with unique biophysical properties. Prog. Lipid Res. 2014, 54, 53-67. |www|
  • Cytotoxic bile acids, but not cytoprotective species, inhibit the ordering effect of cholesterol in model membranes at physiologically active concentrations. Biochim. Biophys. Acta 2013, 1828(9):2152-2163. |www|


Protein-lipid and protein-protein interactions

  • Protein-lipid Interactions
  • Amyloid fiber formation

Representative Publications:
  • Electrostatically driven lipid–lysozyme mixed fibers display a multilamellar structure without amyloid features. Soft Matter2014, 10(6),840-850. |www|
  • Exploring homo-FRET to quantify the oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium. Physical Chemistry Chemical Physics, 2014, 16, 18105-18117. |www|
  • Modeling FRET to investigate the selectivity of lactose permease of Escherichia coli for lipids. Mol. Membr. Biol. 2014, Jun;31(4):120-30. |www|
  • Quantifying lipid-protein interaction by Fluorescence Correlation Spectroscopy (FCS). Methods Mol Biol. 2014, 1076:575-95. |www|
  • Fluorescence detection of lipid-induced oligomeric intermediates involved in lysozyme “amyloid-like” fiber formation driven by anionic membranes. J. Phys. Chem B. 2013, 117(10):2906-17. |www|

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